Vaccination ofChicks with Experimental Newcastle Disease and Avian Influenza Oil-emulsion Vaccines

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چکیده

The immunologie efficacy of inactivated oil-emulsion vaccines against Newcastle disease (ND) and avian influenza (AI) by use of embryo vaccination technology was evaluated. The vaccine antigens were prepared from LaSota strain of ND and H9N2 strain of AI viruses. These antigens were emulsified with an oil adjuvant. 18-day-old embryonated eggs from Aryan broiler breeder were divided into three groups and were delivered inactivated oil-ND and -AI. Group 3 was considered as unvaccinated control. After incubation period, no significant difTerence (P<O.05) was observed in hatchability between two treatrnent and control groups. At intervals of experimental procedure up to 6 weeks the haemgglutinationinhibition antibody titer of groups 1 and 2 were higher than control group. The results of our study indicate that vaccination of chicks against ND and AI vaccines by in ovo technique, were successful under laboratory conditions.

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تاریخ انتشار 2017